Abstract
The effect of increasing cellular ribose 5-phosphate (ribose-5- P) availability by methylene blue-induced acceleration of the oxidative pentose phosphate pathway, on the rate of 5-phosphoribosyl-1-pyrophosphate ( P-ribose- PP) generation, was studied in slices of rat liver at varying P i concentration. It was found that at P i concentration prevailing in the tissue at extracellular physiological P i concentration, ribose-5- P availability is saturating for P-ribose- PP generation, as gauged by the rate of adenine incorporation into tissue nucleotides. The effect of altering P-ribose- PP availability on the rate of de novo purine production gauged by the rate of formate incorporation into purines, was also studied. It was found that the physiological P-ribose- PP concentration in rat liver tissue is limiting for purine synthesis de novo. Depletion of cellular P-ribose- PP, achieved by increase of P-ribose- PP consumption, decelerated purine synthesis, while increase of P-ribose- PP availability, achieved by activation of P-ribose- PP synthetase occurring at elevated P i concentration, resulted in acceleration of purine synthesis.
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Schedule a callMore From: Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis
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