The Effect Of Resistance And Endurance Training On Muscle Proteome Expression In Human Skeletal Muscle

Abstract

PURPOSE: The purpose of the present study was to identify proteins as bio-marker which relate to different types of exercise, using 2-DE and MS. METHODS: To investigate muscle proteome involved in exercise training type, we examined change of proteins expression in vastus lateralis by Two-dimensional gel eletrophoresis and MALDI-TOF MS. Ten physically active young men (23.4±3.2yr, 179.2±3.5cm, 70.1±5.4kg) were trained in two types of exercise (aerobic; n=5/anaerobic; n=5) for 4 weeks. RESULTS: Differential patterns of protein expression were determined after 4 weeks of endurance or resistance exercise training. Following endurance exercise training, carbonic anhydrase III immunoglobulin heavy chain, myosin heavy chain 1, titin, chromosome 12, fructose-1,6-bisphosphatase 2 were up-regulated while pyruvate kinase 3 isoform, ubiquitin carboxyl-terminal hydrolase, phosphoglucomutase were down-regulated. After the 4 weeks of resistance exercise training, five proteins, apolipoprotein A-IV precursor, microtubule-actin crosslinking factor 1, myosin light chain, growth hormone inducible transmembrane protein, unknown protein were up-regulated and pyruvate kinase 3 isoform, human albumin, and enolase 3 were down-regulated. CONCLUSION: We conclude that endurance and resistance exercise training alter differently the expression of individual muscle proteins, and that the response of muscle proteins expression may be associated with specific myofibre adaptations to exercise training. Proteomic studies represent one of the developing techniques of metabolism which may substantially contribute to new insights into muscle and exercise physiology.