The effect of red passion fruit (Passiflora edulis Sims.) fermentation time on its activity against Extended Strain Methicillin-Resistant (ESBL) Escherichia coli and Methicillin-Resistant Staphylococcus aureus (MRSA).

Abstract

The purpose of this study is to determine the effect of fermentation techniques on the inhibitory activity of red passion fruit (Passiflora edulis Sims.) fermentation filtrate in De Man Rogosa Sharpe-broth (MRS-B) media against Extended Strain Methicillin-Resistant (ESBL) Escherichia coli and Methicillin-Resistant Staphylococcus aureus (MRSA). The fruit pulp was wrapped in banana leaves before compared to direct fermentation processes. This study was divided into three treatment groups. Group 1 was the fruit pulp (5g) fermented in 45mL of MRS-B medium for 24h. Group 2 was the fruit pulp wrapped in banana leaves for 3days before fermented in MRS-B for 24h. Group 3 was the fruit pulp wrapped in banana leaves for 3days before fermentation in MRS-B for 48h. Fermentation broth of each condition was taken and then filtered using millipore (0.2µm). As many as 50µL of filtrates was tested for its inhibitory activity against E.coli ESBL and MRSA using the Kirby Bauer method. Group 2 showed the best antibacterial activity against E.coli ESBL and MRSA with the average zone of inhibition of 38.3and 37.6mm respectively. These values were higher than the first and group 3s activities. The inhibitory activity of group 1s against ESBL and MRSA is categorized as a moderate potency with a diameter of growth inhibition zone of 16-20mm, whereas the other groups are categorized as strong potency with a diameter higher than 20mm.