The effect of periodical estradiol-17β injections with different doses on Java barb (Puntius javanicus) gonadal development

Abstract

The aim of research was to determine the effect and the best dosage of estradiol-17β hormone on gonadal development of Java barb (Punctius javanicus). The subjects of this study were 10 months old 48 female (?) Java barb weighing ±200 gram. This research was done experimentally by applying a randomised design (CRD) with 4 treatments and 3 replications. Dosage treatments used were 0, 100, 200, 300 µg/kg body weight. The data observed include body weight, body length, gonado somatic Index (GSI), Gonado Maturation Stage (GMS), hepato somatic index (HSI), total protein plasma (TPP) and water quality. The results showed that the highest body weight was obtained at treatment C 21.42±1.23 g. The highest body length was on treatment C 1.17±0.18 cm. The highest percentage GSI was at treatment C 14.84±0.79%. Meanwhile, the highest GMS were treatment C and D as all samples reached GMS IV on the 30th day. The highest percentage of HSI was at treatment C 2.25±0.36%. Treatment TTP A ranged 4.2-4.6 gr/dL, B 4.4 - 5.4 gr/dL, C 4.4-5.7 gr/dL, and D 4.5-5.8 gr/dL. The water quality of cultivation media was in the proper range at the temperature 26.5-28°C, DO 3-4,1, and pH 7–8. This research concluded that estradiol-17β injection had a significant effect (P<0.05) on body weight gain, GSI, HSI, yet it has not significant effect on body length. The best treatment is treatment C dose of 200 µg/kg body weight.