Abstract
BackgroundThe aim of this study was to optimize the fungal treatment of lignocellulosic biomass by stimulating the colonization. Wheat straw and wood chips were treated with Ceriporiopsis subvermispora and Lentinula edodes with various amounts of colonized millet grains (0.5, 1.5 or 3.0 % per g of wet weight of substrate) added to the substrates. Also, wheat straw and wood chips were chopped to either 0.5 or 2 cm. Effectiveness of the fungal treatment after 0, 2, 4, 6, or 8 wk of incubation was determined by changes in chemical composition, in vitro gas production (IVGP) as a measure for rumen degradability, and ergosterol content as a measure of fungal biomass.ResultsIncomplete colonization was observed for C. subvermispora treated wheat straw and L. edodes treated wood chips. The different particle sizes and amounts of inoculum tested, had no significant effects on the chemical composition and the IVGP of C. subvermispora treated wood chips. Particle size did influence L. edodes treatment of wheat straw. The L. edodes treatment of 2 cm wheat straw resulted in a more selective delignification and a higher IVGP than the smaller particles. Addition of 1.5 % or 3 % L. edodes inoculum to wheat straw resulted in more selective delignification and a higher IVGP than addition of 0.5 % inoculum.ConclusionParticle size and amount of inoculum did not have an effect on C. subvermispora treatment of wood chips. At least 1.5 % L. edodes colonized millet grains should be added to 2 cm wheat straw to result in an increased IVGP and acid detergent lignin (ADL) degradation.
Highlights
The aim of this study was to optimize the fungal treatment of lignocellulosic biomass by stimulating the colonization
The substrates were submerged in water for 3 d after which the excess water was removed by draining through nets and the substrates divided over 1.2 L autoclavable polypropylene containers and covered with a lid containing a filter through which air can pass
It should be noted that the ergosterol contents of pure mycelium grown on malt extract agar may be different for the ergosterol contents of mycelium grown on millet grains
Summary
The aim of this study was to optimize the fungal treatment of lignocellulosic biomass by stimulating the colonization. Wheat straw and wood chips were treated with Ceriporiopsis subvermispora and Lentinula edodes with various amounts of colonized millet grains (0.5, 1.5 or 3.0 % per g of wet weight of substrate) added to the substrates. Cellulose is bound to hemicellulose and lignin in the lignocellulosic complex. Lignin is hardly affected and as a result, the lignocellulosic complex has a limited degradability [2]. Cellulose and hemicellulose in this complex have a limited availability to the rumen microbes. To increase this availability of cellulose, and
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