Abstract
Background and Aim: The Human Fertilization and Embryology Authority declared that assisted hatching (AH) is an unproven add-on intervention. Inline, the guidelines of the National Institute for Clinical Excellence do not recommend the use of AH to improve pregnancy rates. Of note, available data are mainly focused on the effect of AH in fresh embryo transfers while data regarding the effect of laser AH (LAH) on blastocysts, especially after cryopreservation, are inconclusive. This study aimed to investigate the benefits of the laser-mediated partial removal of the zona pellucida in vitrified/warmed blastocysts.
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