The effect of immunomodulation of stimulator antigen presenting cells on subsequent responder T-cell function

Abstract

In this study, we examined whether lipopolysaccharide (LPS)-, interferon-γ (IFN-γ) or 16,16-dimethyl prostaglandin E 2 (dmPGE 2)-pretreatment of stimulator spleen cells from C57BL6 (B6) mice affects effector function of responder T-lymphocyte from C3H HeJ mice. Stimulation of B6-derived splenic mononuclear cells (SMNCs) with LPS (10 μg/ml) prior to their utilization as stimulator cells in a mixed lymphocyte culture (MLC) resulted in an increase in responder T-lymphocyte proliferation compared to utilization of unstimulated SMNC ( P < 0.05). IFN-γ demonstrated similar effects in a dose dependent fashion with maximal stimulatory effect seen at 1000 U/ml. In contrast, pretreatment of stimulator SMNC with dmPGE 2 resulted in dose-dependent inhibition of the responder T-lymphocyte proliferation with maximum inhibitory effect seen using a concentration of dmPGE 2 of 10 −5 M. The presence of indomethacin in the MLC did not reverse this effect. These data demonstrate the effect of immunomodulation of stimulator spleen cells on subsequent T-lymphocyte function.