Abstract
Summary The activities of two isoenzymes of 6-phosphogluconate dehydrogenase from suspension-cultured cells of Catharanthus roseus were not inhibited by 10 μM fructose-2,6-bisphosphate, which has been reported to be a potent inhibitor of 6-phosphogluconate dehydrogenase from castor beans. Slight inhibition was observed when the concentration of this compound was increased to 1 mM. However, this concentration of fructose-2,6-bisphosphate is more than 100 times higher than the estimated concentration in vivo. The activity of the isoenzymes of 6-phosphogluconate dehydrogenase from Catharanthus roseus was inhibited competitively by glucose- 1,6-bisphosphate. The Ki values of two isoenzymes were 190 μM and 175 μM, respectively. These data suggest that inhibition of 6-phosphogluconate dehydrogenase by fructose-2,6bisphosphate is not of great importance in the regulation of the pentose phosphate pathway in Catharanthus roseus cells in vivo .
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