Abstract
Measurements of oxygen uptake were done in the Warburg apparatus by conventional methods (Umbreit, Burris, and Stauffer, 1945), at 37 C, in an atmosphere of air. Flasks of 20-ml capacity contained 2 ml of cell suspension and 0.8 ml of substrate; the center wells contained 0.12 ml of 10% KOH. The values shown for oxygen uptake in the curves are cumulative throughout the experimental period. Cell suspensions were prepared from cultures of Lept. icterohemorrhagiae (Wijnberg strain) which had been grown in a slightly modified Stuart's
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