Abstract
The purpose of this study was to determine if incubation of coronary and pulmonary arteries with dihydrotestosterone (DHT) alters vascular reactivity to potassium chloride (KCl) and the nitric oxide donor 6-(2-hydroxy-1-methyl-2-nitrosohydrazino)-N-methyl-1-hexanamine (NOC-9). Porcine coronary and pulmonary arterial segments were dissected, mounted in tissue baths, and incubated with 0.1 μM DHT for two hours. The responses to increasing concentrations of KCl (5–20 mM) and NOC-9 (10−8–10−5 M) were recorded. To study the expression of the androgen receptor (AR) in the vasculature, Western blots were performed utilizing coronary and pulmonary arteries. Both coronary and pulmonary arteries exhibited significant, dose-dependent contractions in response to KCl and relaxations in response to NOC-9. The vascular reactivity was decreased in coronary arteries and increased in pulmonary arteries following incubation with DHT. The AR is expressed in both coronary and pulmonary arteries. In conclusion, incubation of coronary and pulmonary arteries with DHT may alter vascular reactivity in a heterogeneous manner. This study was supported by a Student Summer Scholars grant at Grand Valley State University.
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