Abstract
Diapause in insects typically results in metabolic adjustments that may include the levels of feeding and activity, metabolic rate, and the accumulation and utilization of reserves. The objective of this study was to characterize and quantify the various neutral lipid classes in the pitcher-plant mosquito (Wyeomyia smithii) that are associated with photoperiod-induced diapause. We used high-performance thin-layer chromatography (HPTLC) with 10 cm × 20 cm silica gel plates, petroleum ether-diethyl ether-glacial acetic acid 80:20:1 as the mobile phase, and 5% ethanolic phosphomolybdic acid solution as detection reagent to determine the percentage of replicate larvae samples (consisting of 16–31 larvae) that contained free sterols (FS), free fatty acids (FFA), triacylglycerols (TG), methyl esters (ME), and steryl esters (SE). Irrespective of the growth treatment (shortdays or long-days), the greatest concentrations of neutral lipids in all samples examined were TG. The effects of day length were such that du...
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
