Abstract
Protease is one of important industrial enzyme produced by microorganisms, including fungi. Aspergillus flavus DUCC K225 is an indigenous mold isolated from lime soil Madura island, has been verified as potential producing protease at pH 8. The production of alkaline protease by A. flavus DUCC K225 was studied under submerged fermentation. Molasse and glucose were used to replace sucrose as carbon source. The effect of medium components was observed on the 7th day’s incubation, by measuring biomass, protease activity, protein content and protease specific activity at pH 8.5. The examination were done for crude enzyme, 0-20%, 20-40%, 40-60%, 60-80% and 80-100% ammonium phosphate fractionation. The results showed that highest biomass found in glucose medium (14,27 g/L); the highest protease specific activity was found in 60-80% ammonium sulfate fractionation of molasses medium (120,17 U/ml) and 9,4 purification fold.
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