The Effect of Chronic Oil Pollution on Salt-Marsh Nitrogen Fixation (Acetylene Reduction)

Abstract

Annual acetylene reduction rates associated with interidal communities in a chronically oil polluted Virginia salt marsh were compared to rates measured in an undisturbed marsh. Chronic oil treatment resulted in visible damage to the higher plants of theSpartina alterniflora zones; however, vegetation-associated acetylene, reduction was not different from the untreated control. Sediment rates generally were affected little by oil application, except during the summer when rates in the median tidal elevation zones were considerably higher than those of the control. Acetylene reduction occurred in all transects, each of which extended from upper mudflat to theSpartina patens zone. Intertidal sediment acetylene reduction was patchy, both spatially and seasonally. Estimated rates were greatest near the surface; free-living bacterial N2 fixation activity averaged 2.23 mg N per m2 per d (range=undetectable to 365 mg N per m2 per d) in the untreated and 3.17 mg N per m2 per d (range=undetectable to 564 mg N per m2 per d) in the oil-treated marsh during the year. Vegetation-associated N2 fixation activity yielded highest overall mean rates (156 mg N per m2 per d). The seasonal pattern of sediment and vegetation-associated fixation may be controlled by temperature and availability of oxidizable substrates.