Abstract
The goal of the study is to evaluate the effect of Radachlorin (OOO “RADA-PHARMA”, Russia) (RC) on platelet aggregation in ex vivo and in vivo experiments. The experiments were conducted on male Wistar rats. Platelet aggregation activity was determined in platelet-rich plasma (PRP) using a turbidimetric method and the aggregation inducer was ADP at a final concentration of 1.25 μM. PRP samples containing RC were irradiated with ALOD-Granat laser device (OOO “Alkom Medika”, Russia) at 662 nm wavelength with 0.05 W/cm2 power density. After a 5-minute incubation of PRP with RC in the dark, dose-dependent inhibition of platelet aggregation was observed. Laser irradiation (12.5 J/cm2 and, especially, 25 J/cm2) increased the inhibitory effect of RC. 3 hours after intravenous administration of RC, the rate and intensity of platelets aggregation did not change, while disaggregation slowed down significantly. Irradiation at a dose of 5 J/cm2 did not affect the platelets aggregation kinetics, and disaggregation slowed down even more at 10 J/cm2, and at 20 J/cm2 the rate and intensity of platelets aggregation decreased, and no disaggregation occurred.In vitro, RC inhibited the ADP-induced platelet aggregation in rats in a dose-dependent manner; after laser irradiation, this effect was enhanced significantly. The effect of RC on circulating platelets leads to a change in their functional state, which manifests in slowing down the disaggregation after exposure to ADP. After laser irradiation (10 J/cm2 and, especially, 20 J/cm2), the severity of the functional changes increases. The role of decreasing the disaggregation activity of platelets in the mechanism of vascular thrombosis in the affected area of photodynamic therapy (PDT) is discussed.
Highlights
The goal of the study is to evaluate the effect of Radachlorin (OOO “RADA-PHARMA”, Russia) (RC) on platelet aggregation in ex vivo and in vivo experiments
* – p
* – p
Summary
Чефу Влияние препарата на основе хлорина е6 на агрегационную активность тромбоцитов. Санкт-Петербург, Россия 2Национальный медицинский исследовательский центр им. Цель исследования – изучение влияния радахлорина на агрегационную активность тромбоцитов в опытах in vitro и ex vivo. Агрегационную активность тромбоцитов определяли в плазме, обогащенной тромбоцитами (PRP), турбидиметрическим методом, индуктор агрегации – аденозиндифосфат (АДФ) в конечной концентрации 1,25 μМ. Через 3 ч после внутривенного введения фотосенсибилизатора скорость и интенсивность агрегации тромбоцитов не изменялись, а дезагрегация значимо замедлялась. В условиях in vitro радахлорин дозозависимо ингибирует АДФ-индуцированную агрегацию тромбоцитов крыс; после лазерного облучения этот эффект значимо усиливается. Воздействие радахлорина на циркулирующие тромбоцитов приводит к изменению их функционального состояния, что проявляется в замедлении дезагрегации после воздействия АДФ. Для цитирования: Петрищев Н.Н., Галкин М.А., Гришачева Т.Г., Дементьева И.Н., Чефу С.Г. Влияние препарата на основе хлорина е6 на агрегационную активность тромбоцитов // Biomedical Photonics. Petrishchev N.N.1,2, Galkin M.A.1, Grishacheva T.G.1, Dementjeva I.N.1, Chefu S.G.1,2 1Pavlov First Saint Petersburg State Medical University, Saint Petersburg, Russia 2Almazov National Medical Research Centre, Saint Petersburg, Russia
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.