Abstract
Chaihu-shugan-san, as a traditional Chinese herbal formula, is composed of seven different herbs. This medicine can treat cancer due to its antioxidant compounds. In this study, the effect of Chaihu-shugan-san was considered on cytotoxicity induction and PDGF gene expression in cervical cancer cell line HeLa at different concentrations and at different times, by the MTT method. Paclitaxel + cisplatin were used as a control in this study. The expression of the PDGF gene was quantitatively evaluated in treated cells by real-time PCR, and a generalized linear model was used to evaluate the effect of the medicine, and Duncan's multiple range tests were used to evaluate the data. The results of the MTT test showed that Chaihu-shugan-san had antitumor properties in different concentrations, but there was a significant difference between this medicine and paclitaxel +cisplatin. Also, examination of gene expression showed that this medicine reduced the expression of the PDGF gene in the HeLa cancer cell line (P ? 0.04). Therefore, Chaihu-shugan-san could be suggested as an effective factor in preventing the growth of cervical cancer cells and controlling angiogenic factors that play an important role in the metastasis of cancerous tumors.
Highlights
Tumor cells are part of the body cells that lose their ability to regulate proliferation and increase indefinitely [1, 2]
Chaihu-shugan-san could be suggested as an effective factor in preventing the growth of cervical cancer cells and controlling angiogenic factors that play an important role in the metastasis of cancerous tumors
The present study showed that Chaihu-shugan-san at concentrations of 10, 100, and 200μg/ml reduced the expression of the PDGF gene
Summary
Tumor cells are part of the body cells that lose their ability to regulate proliferation and increase indefinitely [1, 2] These cells induce the formation of new veins from the existing capillary network through a process that is very similar to natural angiogenesis to supply the oxygen and nutrients they need. Cervical cancer results from the increased and irregular growth of the epithelial cells of the cervix and the constant shedding of these cells. It is the fourth most common cancer and the fourth leading cause of cancer death in women [7]. 70% of cervical cancers occur in developed countries, and about 8% of all cancer deaths belong to it [8]
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