Abstract
Male Sprague-Dawley rats were treated by intratracheal instillation with 1 mg/animal of refractory ceramic fibers. Intratracheal exposure to ceramic fibers led to significant changes of immune response. Results of proliferative activity of spleen lymphocytes showed significantly decreased proliferative activity of T-cells in response to mitogens phytohemagglutinin and concanavalin A in animals given ceramic fibers in comparison with control rats. Similarly, T-dependent B-cell response to pokeweed mitogen was significantly suppressed. Spontaneous proliferative activity of lymphocytes in non-stimulated spleen cell cultures did not differ in exposed and control rats. No significant changes were found among groups in percentage of phagocytic blood polymorphonuclear leukocytes and percentage of cells with respiratory burst.
Highlights
The evidence for adverse health effects following exposure to asbestos has prompted a drastic reduction in the use of asbestos, resulting in the increased use of substitutes composed of both naturally occurring and synthetic materials which are thought to have lower toxicity.Ceramic fibers are important component of insulation wools
After incubation particles were left on shaker for 45 min at room temperature and four times washed in PBS
Intratracheal administration of ceramic fibers to Sprague-Dawley rats resulted in significant changes in immune system of exposed animals
Summary
The evidence for adverse health effects following exposure to asbestos has prompted a drastic reduction in the use of asbestos, resulting in the increased use of substitutes composed of both naturally occurring and synthetic materials which are thought to have lower toxicity.Ceramic fibers are important component of insulation wools. Our study was aimed to assess the effect of ceramic fibers on selected parameters of systemic cellular immune response in Sprague-Dawley rats. In vitro lymphocyte proliferative assay Spleen cells were rinsed out by syringe with needle into complete RPMI cultivate medium with heparin. Male Sprague-Dawley rats (ISASZEG, Hungary) with starting weight of 200–220 g were treated by intratracheal instillation with 1 mg of refractory ceramic fibers (1 mg/ animal, Carbolane Fibres, Saint-Gobain, Ceramiques Industrielles, Nanterre, France).
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Schedule a callMore From: Biomedical papers of the Medical Faculty of the University Palacky, Olomouc, Czechoslovakia
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