Abstract
Burkholderia sp. strain Nafp2/4-1b is a rhizobacterium isolated from the rhizosphere of grassland in South Africa. This draft genome report confirms the presence of genes related to iron acquisition, alleviation of abiotic stress in plants, and other essential traits of plant growth-promoting rhizobacteria (PGPR) that signify the potential of this strain as a plant growth-promoting agent.
Highlights
Burkholderia sp. strain Nafp2/4-1b is a rhizobacterium isolated from the rhizosphere of grassland in South Africa
For DNA extraction, a single pure colony of the bacterium was grown in Luria Bertani (LB) broth for 24 h on a rotary shaker at 28°C
Nafp2/4-1b revealed the presence of the genes that code for the following plant growth-promoting rhizobacteria (PGPR) traits: 1-aminocyclopropane-1-carboxylate (ACC) deaminase, iron-binding pyoverdine siderophore biosynthesis, tryptophan synthase beta and alpha chains involved in auxin biosynthesis, ferric (Feϩ3) hydroxamate siderophore iron transporter (FhuB gene), and ATP binding cassette (ABC) transporters that mediate the uptake of the heavy metalcomplexing peptides iron, siderophores, and phytochelatin
Summary
Burkholderia sp. strain Nafp2/4-1b is a rhizobacterium isolated from the rhizosphere of grassland in South Africa. Based on the growth-promoting traits exhibited in both the gnotobiotic test and the in vitro characterization, the genome of this strain was sequenced to confirm and increase our understanding of its PGPR properties for possible use as a biofertilizer inoculant in crop production. For DNA extraction, a single pure colony of the bacterium was grown in Luria Bertani (LB) broth for 24 h on a rotary shaker at 28°C. One milliliter of the culture suspension was used to extract total DNA using the Wizard genomic DNA purification kit and protocol (Promega, Madison, WI, USA).
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