Abstract
Abstract 1. 1. Asterosaponins were isolated from the aboral body wall, oral body wall, stomach, and pyloric caecum of the starfish Leptasterias polaris . 2. 2. Low-pressure liquid chromatography in conjunction with thin layer chromatography were used to delineate the mixture of saponins. 3. 3. Reverse phase high performance liquid chromatography demonstrated the presence of distinctive mixtures of saponins in the different body components, although similarities were evident in the saponin mixtures from the aboral and oral body walls, and those from the stomach and gonad. 4. 4. The saponin content was determined for each of the body components. The quantity of the saponin in the stomach was significantly higher than in the other tissues which in turn were not significant different from each other. 5. 5. The sugar content and composition was determined for each saponin mixture. Quinovose was the main sugar in all body components except the pyloric caecum where xylose predominated. 6. 6. Asterone and iso-asterone were identified from the mixture of aglycones obtained by acid hydrolysis of the saponin mixture from whole specimens.
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