Abstract
We have measured post-irradiation recovery (potentially lethal damage repair) after fractionated radiation in plateau-phase cultures of two human tumour cell lines derived from tumours of different radiocurabilities (melanoma and breast). Although the radiation survival-curve parameters of these cell lines are similar, the repair of potentially lethal X-ray damage after fractionated X-ray treatment conferred significant radioresistance on the human melanoma cells but not the human breast carcinoma cells. We suggest that the repair of potentially lethal damage may correlate with clinical radiocurability.
Highlights
IONIZING RADIATION has 1959), as well as possible differences in the become an integral part of modern human intrinsic X-ray sensitivity of tumour cells cancer therapy, the biological explanation vs normal tissue (Weichselbaum et al, of therapeutic success or failure remains 1980; Smith et al, 1978; Barendsen, 1980). elusive (Kaplan, 1970, 1974)
A clonally derived human melanoma line (C-143) and a clonally derived human breast carcinoma line (MCF-7) were grown in Eagle's minimal essential medium supplemented with 15% foetal calf serum, 900 mg/l of glucose, 0-6 delayed, an enhancement in survival mg/l of sodium pyruvate and 15 mg/l of occurs
The differences in the responses of the two cell lines is evident in Fig. 4, in which survival in the C-143 melanoma and MCF7 cells are compared after fractionated irradiation with 24 h recovery after the last dose
Summary
A clonally derived human melanoma line (C-143) and a clonally derived human breast carcinoma line (MCF-7) were grown in Eagle's minimal essential medium supplemented with 15% foetal calf serum, 900 mg/l of glucose, 0-6 delayed, an enhancement in survival mg/l of sodium pyruvate and 15 mg/l of occurs. Recovery was measured as the enhancement in survival melanoma lines was unusually resistant to as measured by colony-forming ability resultthe lethal effects of single doses of X-rays ing from the delay in subculture; it is plotted (survival curve Do=2' 11 Gy), the Do for the osteosarcoma line and other melanoma line ranged between 1-40 and 1-50 Gy-well within the range of normal cells (Weichselbaum et al, 1982). The doses were chosen so that the 0 h (initial surviving) level would be equivalent for both cell types
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