The Differential Profiles of Withdrawal Symptoms and Regulation of Signal Molecules and Gene Expressions Induced by i.c.v. Morphine and Beta-Endorphin

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Event Abstract Back to Event The Differential Profiles of Withdrawal Symptoms and Regulation of Signal Molecules and Gene Expressions Induced by i.c.v. Morphine and Beta-Endorphin Soo-Hyun Paks1*, Yun-Beom Sim1, Seung-Min Choi1, Jin-Koo Lee1 and Hong-Won Suh1 1 Institute of Natural Medicine, College of Medicine, Hallym University, Medical and Bio-Materials Research Center and Department of Pharmacology, South Korea In this study, withdrawal symptoms induced by morphine (MOR) and beta-endorphin (BEND) administered i.c.v. were compared in ICR mice. In addition, the differential regulation of signal transduction molecules and several gene expressions were assessed in the various brain regions. To perform, naloxone (NAL) was post-treated i.p. 3 hours after either a single or repeated (1 time/day for 3 days) injection with MOR or BEND. Withdrawal symptoms were observed for 30 min immediately after NAL treatment. The jumping, diarrhea, weight loss, rearing, penile licking and paw tremor observed in NAL/MOR treated group was persistently increased during 3 days. On the other hand, the diarrhea, weight loss and rearing induced by NAL/BEND treatment were increased after single injection with BEND, but gradually decreased after repeated injection with BEND. Furthermore, no jumping, penile licking and paw tremor in NAL/BEND treated group were observed throughout whole period of time. In molecular assay, various brain regions were dissected each at 30min, 1 hour and 2 hours after NAL treatment. The POMC, CRH and c-Fos gene expressions may be involved both the supraspinal MOR or BEND treatment and withdrawal in various brain regions. In addition, acute or chronic MOR or BEND treatment and withdrawal were regulated by nearly similar signal transduction molecules mechanisms, but chronic BEND withdrawal could not modulate the pCaMK-II activity in the various brain regions. These results suggest that withdrawal symptoms profiles of MOR or BEND administered supraspinally appear to be differentially regulated. Furthermore, the acute and chronic MOR or BEND treatment and withdrawal were regulated by nearly similar signal transduction molecules mechanisms and gene expressions. On the other hand, supraspinally repeated treatment-induced chronic withdrawal state of MOR or BEND was differential regulated the Ca2+-dependent signal-regulated molecules such as pCaMK-II in the various brain regions. Conference: IBRO International Workshop 2010, Pécs, Hungary, 21 Jan - 23 Jan, 2010. Presentation Type: Poster Presentation Topic: Cognition and behavior Citation: Paks S, Sim Y, Choi S, Lee J and Suh H (2010). The Differential Profiles of Withdrawal Symptoms and Regulation of Signal Molecules and Gene Expressions Induced by i.c.v. Morphine and Beta-Endorphin. Front. Neurosci. Conference Abstract: IBRO International Workshop 2010. doi: 10.3389/conf.fnins.2010.10.00178 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 30 Apr 2010; Published Online: 30 Apr 2010. * Correspondence: Soo-Hyun Paks, Institute of Natural Medicine, College of Medicine, Hallym University, Medical and Bio-Materials Research Center and Department of Pharmacology, Chuncheon, South Korea, shyun1017@hanmail.net Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Soo-Hyun Paks Yun-Beom Sim Seung-Min Choi Jin-Koo Lee Hong-Won Suh Google Soo-Hyun Paks Yun-Beom Sim Seung-Min Choi Jin-Koo Lee Hong-Won Suh Google Scholar Soo-Hyun Paks Yun-Beom Sim Seung-Min Choi Jin-Koo Lee Hong-Won Suh PubMed Soo-Hyun Paks Yun-Beom Sim Seung-Min Choi Jin-Koo Lee Hong-Won Suh Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.