Abstract
Aflatoxin B1 (AFB1) is a naturally occurring carcinogenic and immunosuppressive compound. This study was designed to measure its toxic effects on human peripheral blood mononuclear cells (PBMC). The study recruited 7 healthy volunteers. PBMC were isolated and cellular respiration was monitored using a phosphorescence oxygen analyser. The intracellular caspase activity was measured by the caspase-3 substrate N-acetyl-asp-glu-val-asp-7-amino-4-methylcoumarin. Phosphatidylserine exposure and membrane permeability to propidium iodide (PI) were measured by flow cytometry. Cellular oxygen consumption was inhibited by 2.5 μM and 25 μM of AFB1. Intracellular caspase activity was noted after two hours of incubation with 100 μM of AFB1. The number of Annexin V-positive cells increased as a function of AFB1 concentration and incubation time. At 50 μM, a significant number of cells became necrotic after 24 hours (Annexin V-positive and PI-positive). The results show AFB1 is toxic to human lymphocytes and that its cytotoxicity is mediated by apoptosis and necrosis.
Highlights
Aflatoxin B1 (AFB1) is a naturally occurring carcinogenic and immunosuppressive compound
Application to Patient Care - The cytotoxicities of aflatoxin in humans include immunosuppression, mediated by lymphocyte apoptosis and necrosis. - Public awareness of the potential immunotoxicity of aflatoxins is needed. - Effective health regulations are required to minimise the exposure to aflatoxins, especially in immunocompromised hosts
This study explores the mechanisms of the toxic effects of AFB1 in human lymphocytes
Summary
Abstract: Objectives: Aflatoxin B1 (AFB1) is a naturally occurring carcinogenic and immunosuppressive compound. This study was designed to measure its toxic effects on human peripheral blood mononuclear cells (PBMC). Results: Cellular oxygen consumption was inhibited by 2.5 μM and 25 μM of AFB1. The number of Annexin V-positive cells increased as a function of AFB1 concentration and incubation time. Conclusion: The results show AFB1 is toxic to human lymphocytes and that its cytotoxicity is mediated by apoptosis and necrosis. وك شف ن شاط انزيم الكا سبي س داخل الخلية با ستخدام المادة الف سفورية الم شتقة من كومارين القادرة.الخلوي بوا سطة الم ؤ شر الف سفوري لك شف لاأك سجين. - AFB1 induces apoptosis and necrosis in human lymphocytes. - AFB1 inhibits lymphocyte respiration (mitochondrial oxygen consumption). Application to Patient Care - The cytotoxicities of aflatoxin in humans include immunosuppression, mediated by lymphocyte apoptosis and necrosis. Application to Patient Care - The cytotoxicities of aflatoxin in humans include immunosuppression, mediated by lymphocyte apoptosis and necrosis. - Public awareness of the potential immunotoxicity of aflatoxins is needed. - Effective health regulations are required to minimise the exposure to aflatoxins, especially in immunocompromised hosts
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