Abstract
The affinity of BIUL (bismuth iodide impregnation, combined with uranyl acetate and lead hydroxide poststaining) to nerve ultrastructure is examined. Maximal electron microscopic contrast is seen in chromatin, ribosomes, inner and outer fuzz coats of membranes, and especially the membrane complexes at intercellular junctions. Basement lamina and osmiophilic leaflets of unit membranes attain low contrast properties. The BI component reacts with basic amino residues (in vitro studies), while U followed by L not only stains the acidic groups but is apparently bound to BI. Methylation and acetylation experiments support the notion that the high contrast of synaptic membrane complexes as demonstrated by the BIUL method may be related to the presence of high concentrations of anionic as well as cationic groups.
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