Abstract
Comparative proteomics of the multiple strains within the same species can reveal the genetic variation and relationships among strains without the need to assess the genomic data. Similar to comparative genomics, core proteome and pan proteome can also be obtained within multiple strains under the same culture conditions. In this study we present the core proteome and pan proteome of four epidemic Salmonella Paratyphi A strains cultured under laboratory culture conditions. The proteomic information was obtained using a Two-dimensional gel electrophoresis (2-DE) technique. The expression profiles of these strains were conservative, similar to the monomorphic genome of S. Paratyphi A. Few strain-specific proteins were found in these strains. Interestingly, non-core proteins were found in similar categories as core proteins. However, significant fluctuations in the abundance of some core proteins were also observed, suggesting that there is elaborate regulation of core proteins in the different strains even when they are cultured in the same environment. Therefore, core proteome and pan proteome analysis of the multiple strains can demonstrate the core pathways of metabolism of the species under specific culture conditions, and further the specific responses and adaptations of the strains to the growth environment.
Highlights
Over 2500 serotypes have been reported in Salmonella, and most of them result in diarrhea
Any spot detected in all four strains was considered to be a core protein, and the total number of core proteins identified was 739 and 318 within the ranges of pH 4–7 and pH 6–11
The core proteins covered from 85.9%–87.0% of the spots within the range of pH 4–7 and 81.8%–89.6% of the spots within the range of pH 6–11 in each strain, which suggested a high similarity in the protein expression among S
Summary
Over 2500 serotypes have been reported in Salmonella, and most of them result in diarrhea. Within these serotypes, Salmonella enterica serovar Typhi and Paratyphi, can lead to systemic infections in humans, known as typhoid and paratyphoid fever. Salmonella enterica serovar Typhi and Paratyphi, can lead to systemic infections in humans, known as typhoid and paratyphoid fever These diseases cause epidemics in Asia, Africa and Latin America [1,2]. Typhi was the main causative agent of enteric fever in southeast Asia and in China, but in the mid-1990s, the number of cases caused by S. Paratyphi A started to increase, and paratyphoid fever subsequently became the major enteric fever [3,4,5,6]
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