Abstract
This work evaluated the effect of secondary bacterial metabolites produced by Pseudomonas sp LV strain in control of Lactobacillus sp. population in the microcosm of the vat during ethanol fermentation. The fraction F4 produced by Pseudomonas aeruginosa was extracted with dichloromethane and fractionating by vacuum liquid chromatography obtained in a methanol phase. The evaluation of antibiotic activity of F4 fraction mixed or not with sulphuric acid and Kamoram?. The antibiotic activity of F4 fraction was determined as well as the fermentation efficiency. Also was determined yeast cell viability, budding formation, the viability of budding cells, and number of populations of Saccharomyces cerevisiae and Lactobacillus sp. The results showed that the F4 fraction had high selective antibiotic activity against Lactobacillus sp. but not for S. cerevisae, and no inhibitory effect was observed in the fermentation process by yeast. Also F4 fraction decreased flocculation and foam formation. The F4 has an antibiotic activity against Lactobacillus sp. and should be used as an alternative to control bacteria contamination and foam and flocculation formation in the fuel ethanol fermentation process. The F4 fraction could reduce the use of antibiotics in the control of Lactobacillus sp. population during the fuel ethanol production.
Highlights
The sugar cane (Saccharum officinarum) culture is largely used to produce sugar and fuel ethanol
The results showed that the F4 fraction had high selective antibiotic activity against Lactobacillus sp. but not for S. cerevisae, and no inhibitory effect was observed in the fermentation process by yeast
Few studies tested the effect of secondary metabolites produced by bacteria in the control of contaminants microorganisms during the fuel ethanol production [27]
Summary
The sugar cane (Saccharum officinarum) culture is largely used to produce sugar and fuel ethanol. A variety of Gram-positive and Gram-negative bacteria have been isolated from fuel ethanol fermentations including species of Pediococcus, Enterococcus, Acetobacter, Gluconobacter, and others [3,4]. During fuel ethanol production the contamination level can increase the gum formation which can obstruct pipes, sieves, centrifuge, heat sinks, increasing the flocculation of yeast, decreasing fermentation process activity, and losing yeast cells for pellet formation on the fermentator depth. The flocculation decreases the centrifugation efficiency reducing the yeast viability and/or increases a formation of compounds which decreases fermentation activity and ethanol production [5]. The formation of organic acid decreases ethanol production and unfeasible yeast, resulting in an operational problem in the industry facilities [6]
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