Abstract
1. 1. Oxygen evolution by reconstituted chloroplasts with 3-phosphoglycerate as substrate was inhibited by ADP. This inhibition was overcome by increased concentrations of 3-phosphoglycerate or by the addition of excess ATP. 2. 2. The initial rate of 3-phosphoglycerate reduction by chloroplast stromal extracts, measured as 3-phosphoglycerate-dependent oxidation of NADPH, was also dependent on the concentrations of 3-phosphoglycerate, ADP and ATP. 3. 3. Within the range of concentrations of 3-phosphoglycerate, ADP and ATP expected to occur within intact chloroplasts, the rate of 3-phosphoglycerate (PGA) reduction by stromal extract was proportional to the ratio [ATP] [PGA]/[ADP]. 4. 4. These results are consistent with the notion that the substrates and products of 3-phosphoglycerate kinase control the rate of 3-phosphoglycerate reduction via a mass action effect.
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