Abstract
Microsatellite (SSR) markers with known precise intrachromosomal locations are widely used for mapping genes in rye and for the investigation of wheat-rye translocation lines and triticale highly demanded for mapping economically important genes and QTL-analysis. One of the sources of novel SSR markers in rye are microsatellites transferable from the wheat genome. Broadening the list of available SSRs in rye mapped to chromosomes is still needed, since some rye chromosome maps still have just a few microsatellite loci mapped. The goal of the current study was to integrate wheat EST-SSRs into the existing rye genetic maps and to construct a consensus rye microsatellite map. Four rye mapping populations (P87/P105, N6/N2, N7/N2 and N7/N6) were tested with CFE (EST-SSRs) primers. A total of 23 Xcfe loci were mapped on rye chromosomes: Xcfe023, -136 and -266 on chromosome 1R, Xcfe006, -067, -175 and -187 on 2R, Xcfe029 and -282 on 3R, Xcfe004, -100, -152, -224 and -260 on 4R, Xcfe037, -208 and -270 on 5R, Xcfe124, -159 and -277 on 6R, Xcfe010, -143 and -228 on 7R. With the exception of Xcfe159 and Xcfe224, all the Xcfe loci mapped were found in orthologous positions considering multiple evolutionary translocations in the rye genome relative to those of common wheat. The consensus map was constructed using mapping data from the four bi-parental populations. It contains a total of 123 microsatellites, 12 SNPs, 118 RFLPs and 2 isozyme loci.
Highlights
Several linkage maps of rye carrying RFLP, AFLP, SSR, DArT and SNP markers are available to date (Devos et al, 1993; Philipp et al, 1994; Senft, Wricke, 1996; Korzun et al, 2001; Bednarek et al, 2003; Hackauf, Wehling, 2003; Khlestkina et al, 2004; Varshney et al, 2007; Bolibok-Brągoszewska et al, 2009; Gustafson et al, 2009; Milczarski et al, 2011, 2016; Xu et al, 2012; Bauer et al, 2017)
SSR markers were used for mapping the sy1, sy9, sy18 and sy19 asynaptic genes (Malyshev et al, 2009; Dolmatovich et al, 2013a, b), the gene mo1 for supernumerary spikelets (Dobrovolskaya et al, 2009), several anthocyanin biosynthesis genes (Khlestkina et al, 2009, 2011, 2013), Ddw1 (Tenhola-Roininen, Tanhuanpää, 2010) and Ddw3 (Yang et al, 2018) dwarfing genes, the powdery mildew resistance locus (Wang et al, 2010), the Elm-R1 gene related with embryo lethality in wheat-rye hybrids as well as the hybrid dwarfness gene Hdw-R1 (Tikhenko et al, 2011; Tsvetkova et al, 2018), aluminum tolerance loci in rye and triticale (Fontecha et al, 2007; Benito et al, 2010; Niedziela et al, 2014) and several QTL for agronomic traits including grain yield (Hackauf et al, 2017)
SSRs can be suitable for marker-assisted breeding (Lapitan et al, 2007), detection of the genetic variability in rye and triticale (Bolibok et al, 2005; Vyhnánek et al, 2009) as well as for marker-assisted identification of rye genetic material in wheat cultivars and lines (Silkova et al, 2006; Schlegel, Korzun, 2008; Schneider, Molnár-Láng, 2009; Adonina et al, 2011; Silkova et al, 2011; Schlegel, 2015)
Summary
Several linkage maps of rye carrying RFLP, AFLP, SSR, DArT and SNP markers are available to date (Devos et al, 1993; Philipp et al, 1994; Senft, Wricke, 1996; Korzun et al, 2001; Bednarek et al, 2003; Hackauf, Wehling, 2003; Khlestkina et al, 2004; Varshney et al, 2007; Bolibok-Brągoszewska et al, 2009; Gustafson et al, 2009; Milczarski et al, 2011, 2016; Xu et al, 2012; Bauer et al, 2017).SSRs (microsatellites) are among the most widely used DNA-markers in rye genetics. Several linkage maps of rye carrying RFLP, AFLP, SSR, DArT and SNP markers are available to date (Devos et al, 1993; Philipp et al, 1994; Senft, Wricke, 1996; Korzun et al, 2001; Bednarek et al, 2003; Hackauf, Wehling, 2003; Khlestkina et al, 2004; Varshney et al, 2007; Bolibok-Brągoszewska et al, 2009; Gustafson et al, 2009; Milczarski et al, 2011, 2016; Xu et al, 2012; Bauer et al, 2017). The goal of the current study was to integrate wheat EST-SSRs, expressed sequence tag SSR (from map of L.Y. Zhang et al (2005)) into the existing rye microsatellite map and construct the consensus microsatellite map of rye genome
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