Abstract
The inhibitory effect of dicoumarol on the mixed function oxidase system of liver microsomes from phenobarbital treated rats were studied. Lineweaver-Burk plots of p-nitroanisole O-demethylation were non-linear in the presence of dicoumarol but linear in its absence. At high concentrations of p-nitroanisole the inhibition was partially overcome indicating a competition between the substrate and dicoumarol for the enzyme. Furthermore, it was found that dicoumarol promotes a time and concentration dependent conversion of cytochrome P-450 to P-420 and that it inhibits cytochrome P-450 reduction. While these fenomena seem directly related to the inhibitory action of dicoumarol on the overall p-nitroanisole demethylase reaction, an inhibitory effect of dicoumarol on NADH : ferricyanide reductase, on the contrary, was shown not to be related to the inhibition of the demethylation process.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
