Abstract
Pseudomonas putida has attracted much interest for its environmental, industrial, biotechnological, and clinical importance. Here, we report the complete genome sequence of the type strain P. putida NBRC 14164. This genome sequence will assist to further elucidate the molecular mechanisms of the characteristic traits among strains belonging to the species P. putida.
Highlights
Pseudomonas putida has attracted much interest for its environmental, industrial, biotechnological, and clinical importance
The genome of P. putida type strain NBRC 14164 was sequenced by using a combined strategy of shotgun sequencing with 454 GS FLX Titanium (Roche) (106.9-Mb sequences, 17.4-fold coverage) and paired-end sequencing with HiSeq 1000 (Illumina) (572.6-Mb sequences, 93.0-fold coverage)
The genome of strain NBRC 14164 consists of a 6,156,701-bp circular chromosome with a 62.3% GϩC content and contains 5,449 coding sequences (CDSs), 7 copies of rRNA operons, and 74 tRNA genes
Summary
Pseudomonas putida has attracted much interest for its environmental, industrial, biotechnological, and clinical importance. Despite the increasing number of projects that are sequencing this species, the genome of the type strain has not been sequenced (http://www.genomesonline.org/). The genome of P. putida type strain NBRC 14164 was sequenced by using a combined strategy of shotgun sequencing with 454 GS FLX Titanium (Roche) (106.9-Mb sequences, 17.4-fold coverage) and paired-end sequencing with HiSeq 1000 (Illumina) (572.6-Mb sequences, 93.0-fold coverage).
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