Abstract
Micromanipulation method was combined with the AmpliGrid LV-PCR system to select and detect single cells, in order to provide a possible solution for biological mixtures and trace samples. Three fresh buccal cells could be completely genotyped by two STR kits. Sixty parallel single cell LV-PCRs were performed using Identifiler ®, 13 complete profiles (21.7%) and 13 acceptable profiles (13–15 loci) were obtained. Seventy single cells were typed by MiniFiler ®, showing 48.6% full profiles and 18.6% acceptable profiles (6–8 loci). Two mock casework samples, cup and chewing gum, were assayed by single cell LV-PCR using MiniFiler ®. Three out of four repeat experiments for the cup sample were fully genotyped. Twice of four chewing gum experiments yielded complete profiles. We re-analyzed one casework sample which had been shown to be a mixed profile tested by routine method, while single-person profile was obtained by the new method. These results showed great promise for mixtures and trace DNA analysis. Successful capture of intact cell is the key to the experiment. Replicate experiments are also necessitated to get reliable profiles.
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