The clinical application of vascular endothelial growth factor and nuclear transcription factor on diagnosis and prognosis of benign and malignant salivary gland pleomorphic adenoma

Abstract

Objective To investigate the clinical application of vascular endothelial growth factor (VEGF) and nuclear transcription factor(NF-κB)/p50 on diagnosis and prognosis of the abundance of matrix and rich cell type salivary gland pleomorphic adenoma.Methods Fifty cases with parotid pleomorphic adenoma pathological from Jan.2004 to Nov.2004 collected at Affiliated Hospital of Hebei United University and Kailuan General Hospital were selected in this study.There were 25 samples with pleomorphic adenoma of matrix-based abundant salivary gland served as group A,25 samples were cell-based abundant salivary gland pleomorphic adenoma served as group B,and 25 samples were normal salivary gland tissue adjacent to tumor served as control group.The immunohistochemical technology and SP method were applied to detect the expression of VEGF and NF-κB/p50.Results Expressions of VEGF in group A and B were (153.13 ± 60.81) and (954.65 ± 305.79),significantly higher than that in group C (52.46 ± 9.76,P ＜ 0.05).Expressions of NF-κB/p50 in group A and B were (43.40 ± 5.56),(529.79 ± 163.81),significantly higher in that in group C (6.83 ± 1.90,P ＜ 0.05) ; Expressions of VEGF and NF-κB/p50 in group B were higher than that in group A (P ＜ 0.01).There were the positive correlation between EGF and NF-κB/p50 expression in pleomorphic adenoma samples (r =0.9123,P =0.001).Conclusion With the increase in tumor cell components,angiogenesis and cell proliferation activity increase in cases with pleomorphic adenoma.Cases with cell-based abundant pleomorphic adenoma show the malignant transformation tendency compared with cases with matrix-based abundant pleomorohic adenoma. Key words: Salivary gland pleomorphic adenoma; Immunohistochemical; Vascular endothelial growth factor; Nuclear transcription factor