Abstract
The CO 2/HCO 3 − buffering system is one of the main mechanisms implicated in cytosolic pH (pH i) regulation. We studied this pH i-regulatory system in rat mast cells using a fluorescent dye. Mast cells had a more alkaline pH i in the presence of HCO 3 − than in its absence. The recovery from an acid load was faster in HCO 3 −-free conditions than in HCO 3 −-containing media. In HCO 3 −-buffered conditions the increase of the recovery rate of an acidification in 4,4′-diisothiocyanostilbene-2,2′-disulfonic acid-incubated cells suggested the implication of a Na +-independent Cl −/HCO 3 − exchanger. This HCO 3 − transport acidified the cytosol and was also partially responsible for the recovery of intracellular alkalinizations. Moreover, regulation of the recovery rate of an acidification by protein kinase C and calcium signaling pathways depended on the presence or absence of HCO 3 −. The presence of HCO 3 − limits the recovery of acute intracellular acidifications probably through the Na +-independent Cl −/HCO 3 − exchanger and modulates the regulation of pH i by protein kinase C and calcium.
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