Abstract
The goal of this project is to create an optimal environment for wound healing. Preliminary results from a rabbit model suggest that the addition of mesenchymal stromal cells (MSC) to a repaired incision improves the biomechanical properties of the wound. To increase sample size and reproducibility, these studies will be repeated in an inbred rat model. A review of the literature shows a wide variety of culture conditions used for rat MSCs. This study compares proliferation of rabbit and rat MSCs when grown in ten different growth medias. The Cell Titer 96 ® Cell Proliferation Assay and direct cell counts were used to measure the proliferation of MSC's. Rabbit and rat MSC growth was optimal in alpha MEM + 20% FCS or Hams F12 + 10% FCS. To prepare for the extension of these studies into the rat model, four mesh materials commonly used in hernia repair were tested to determine the best vehicle for the addition of MSCs. MSCs were incubated with the mesh for 30 min at 37oC. Non‐adherent cells were removed and the remaining cell number determined by incubation with Cell Titer 96 ® solution and comparison to an MSC standard curve. Optimal adherence was seen with Bard ® CollaMend (38% of added cells). Future studies will use Bard ® CollaMend as a vehicle when adding MSCs to a repaired abdominal incision. Supported by St. Elizabeth Health Center and Youngstown State University.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
