Abstract
In this study, HL-60, a human granulocyte cell line was differentiated into granulocyte-like cell by DMSO and then the change of the amount of Fc receptors and chemotaxis-associated antigen (TM 316) was examined using monoclonal antibodies and fluorescence analysis of cell sorter. The results showed that the amount of chemotaxis-associated antigen increased slightly and chemotactic activity was poor even after HL-60 was well-differentiated. Fc gamma R I (CD 64) and Fc gamma R II (CD 32) receptors in differentiated HL-60 were slightly recognized. However, there was no Fc gamma R III B (CD 16 b) receptor in differentiated HL-60 cells. Although DMSO induced HL-60 was morphologically similar to peripheral granulocytes, differentiated HL-60 was different from peripheral granulocytes in terms of chemotaxis and surface receptors.
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Schedule a callMore From: Nihon Rinsho Men'eki Gakkai kaishi = Japanese journal of clinical immunology
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