The cerebrospinal fluid values of advanced oxidation protein products and total thiol content in patients with amyotrophic lateral sclerosis

Abstract

ObjectivesAmyotrophic lateral sclerosis (ALS) is recognized as a progressive neurodegenerative disorder of unknown origin. Oxidative stress (OS) is considered as one of the most challenging hypothesis in the disease pathogenesis. The aim of this study was to contribute to the understanding of what extent there is involvement of OS in ALS. Patients and methodsWe assessed Advanced Oxidation Protein Products (AOPP) and total thiol (-SH) groups in cerebrospinal fluid (CSF) of 24 ALS patients (13 of them presented with spinal form while 11 patients had bulbar form) and 20 controls (CG). ResultsThe obtained AOPP levels in ALS patients were higher than those in CG (p <0.001), while −SH groups showed lower values compared to CG (p<0.001). The AOPP values were higher in ALS patients with bulbar compared with ALS patients with common spinal manifestation (p<0.001). There were no differences in −SH group’s levels among these different clinical forms (p>0.05). The negative correlation between AOPP and the levels of −SH groups was confirmed (p <0.01). Significant mild correlations between tested parameters and functional rating scale as well as disease progression index were recorded for both of tested parameters in spinal form of ALS (p<0.01). ConclusionThe data presented here clearly support the fact that OS is involved in patophysiology of ALS, where oxidation of −SH groups represents an important aspect of protein oxidation. The CSF AOPP level and −SH groups may serve as potential useful biomarker for functional disorder and progression of the disease in the spinal form of ALS.