The biological changes of autogenous skull flap after subcutaneous preservation and early cranioplasty

Abstract

Objective To investigate the biological changes of autogenous skull flap after subcutaneous preservation and early cranioplasty.Methods The New Zealand white rabbits were divided into 3 groups:normal controls,sham-operated controls and operated groups,and the models of skull flap-removed decompression and subcutaneous preservation were established.The autogenous eamioplasty was clone two weeks after sabeutaneous preservation.The calcium content of skull flap was deterrainated at different time points,and histological and bio-structural changes were observed by using immunohistochemistry stain and electron microscopy scanning.Results The calcium contents in normal and subcutaneously preserved skull flaps were(935.15±166.36)(normal),(847.40±126.97)(at first week),(766.55±126.89)(at 2nd week),(603.53±37.10)(at 4th week),and(444.68±48.05)μg/g(at 8th week)respectively.The calcium contents in the skull flaps after carnioplaty were(628.53±47.16)(at 2nd week),(691.55± 50.61)(at 4th week),and(835.89± 94.75)μg/g(at 8th week)respectively.The skull flaps subcutaneously preserved were wrapped up by fibrous tissue gradually,the marrow cavity was destroyed,and the lacunar ceils in the osseous lamella were dead,but the bone trabecula still existed.After the transplantation of the autogenous skull flap,blood vessels were grown into the bone edges as the time went on,and part of the dead bone reactivated.Conclusion The autogenous skull flap cranioplasy sbould be clone at the 4th week after subcutaneous preservation.If the autogenous skull flap eranioplasy could be done early,the biochemical characters of the skull flaps which preserved subcutaneously could achieve almost normal leve]. Key words: Autogenous bone flap; Cranial bone forming operation; Bone biology