Abstract
1. 1. Histories and masking acidic proteins were removed from hypothalamic chromatin in order to evaluate/measure the number of available acceptor sites for the [ 3H]oestradiol-receptor complex. This number increases after dehistonizing and unmasking and is lower than published values for comparable preparations. 2. 2. No sex-related difference in [ 3H]oestradiol-receptor binding to hypothalamic chromatin in ritro was observed. 3. 3. Failure to observe such a difference suggests that sexual differentiation and steroid sensitivity cannot be attributed to marked differences in the degree of chromatin masking.
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