The Basigin‐variant‐2 binding domain in the Ig0 domain of Basigin‐variant‐1 stimulates an immune response in the mouse monocyte RAW 264.7 cell line

Abstract

There are two main protein products of the Basigin gene. One protein, known as Basigin‐variant‐1 is expressed in the neural retina, specifically by the photoreceptor neurons. The other protein, known as Basigin‐variant‐2 is expressed throughout the body, including monocytes, as well as Müller glial cells and the retinal pigmented epithelium of the eye. A study by this laboratory indicates that the two Basigin gene products interact via their extracellular domains. A different study by another research group indicates that the Ig0 domain of Basigin‐variant‐1 can elicit an immune response in several cell lines. The purpose of the present study was to determine if the region of the Ig0 domain of Basigin‐variant‐1 thought to interact with Basigin‐variant‐2 is the same region that elicits an immune response. Recombinant versions of the Basigin‐variant‐1 Ig0 domain were incubated with mouse monocytic RAW 264.7 cells. After 24 hours, the cell culture medium was collected and assayed for the expression of the proinflammatory cytokine interleukin‐6 (IL‐6) via an ELISA. Cells treated with a control protein generated from the expression vector used to make the Basigin‐variant‐1 recombinant proteins served as the controls. The data indicate that the region of the Ig0 domain used to interact with Basigin‐variant‐2 does indeed stimulate production of a significantly greater amount of IL‐6 than the control protein. The data suggest that Basigin‐variant‐1 interacts with Basigin‐variant‐2 to stimulate a proinflammatory response in monocytes. This study is important for a better understanding of immune response aspects of diabetic retinopathy and other diseases in which the retinal pigmented epithelium is compromised.Support or Funding InformationUNF Office of Academic AffairsThis abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal.