Abstract
The Aim: To evaluate the association of TSHR gene polymorphism rs2268458 with some Physio-biochemical parameters among hypothyroidism women.
 Methods: This study included 51 hypothyroidism women and 43 healthy women as a control group. Some Physio-biochemical (body mass index (BMI), cytochrome 450 1A1 (CYP1A1), calcium, and phosphorus) and hormonal assay (Osteopontin (OPN), thyroid stimulating hormone (TSH), fT3, fT4 and Calcitonin (CT)) assay were performed. Genotyping of rs-2268458 of TSHR gene was carried out using the polymerase chain reaction-based restriction fragment length polymorphism (PCR-RFLP) technique.
 Results: TSHR rs2268458 was associated with hypothyroidism in Iraqi women and allele C can be a risk factor for disease by OR= 4.393, CI=1.42-13.53 where TC and CC genotypes were associated with high BMI, while TC genotype was associated with high calcitonin and CYP1A1 levels.
 Conclusion: The TSHR rs2268458 polymorphism was associated with hypothyroidism in Iraqi women and allele C can be a risk factor for some Physio-biochemical and hormonal disorder in hypothyroidism women.
Highlights
Hypothyroidism is an endocrine disorder defined as a deficiency in thyroid hormone production [1]
Genotyping of TSHR rs 2268458 The results of TSHR gene amplification showed that the amplification product was 162 bp for patients and control as explained in the figure (4)
The result of the study showed that there was no association between TSHR rs2268458 and thyroid function tests (TSH, ft3, and ft4) but there was a significant difference between the genotypes of this SNP and each of the following: body mass index (BMI), CT, and CYP1A1, where TC and CC genotypes were associated with high BMI, while TC genotype was associated with high CT and CYP4501A1 levels, as shown in table (5)
Summary
Hypothyroidism is an endocrine disorder defined as a deficiency in thyroid hormone production [1]. The result of the study showed that there was no association between TSHR rs2268458 and thyroid function tests (TSH, ft3, and ft4) but there was a significant difference between the genotypes of this SNP and each of the following: BMI, CT, and CYP1A1, where TC and CC genotypes were associated with high BMI, while TC genotype was associated with high CT and CYP4501A1 levels, as shown in table (5).
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