Abstract
For the complete resolution and determination of the β-blocker atenolol and its manufacturing impurities a high-performance liquid chromatographic method is developed using structured optimization techniques. The method utilizes a 300 × 3.9 mm i.d. stainless steel column packed with μBondapak C 18 and a mobile phase containing methanol—25 mM potassium dihydrogen orthophosphate containing 0.06% (v/v) dibutylamine (20:80, v/v) (pH 3.0). The flow rate of 1.0 ml min −1 is used and a detection wavelength of 226 nm. The linearity and repeatability are good for the present compound over the range 1.5–510.0 μg ml −1 ( r > 0.99 and RSD 0.27%, n = 10). Application of the method to 50 and 100 mg tablets gave recoveries of 99% (w/w) and reproducibilities of (RSD) 1.1 and 0.52%, respectively ( n = 5). The manufacturing impurities are found to be >0.5% (w/w) of the atenolol peak.
Published Version
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