Abstract
A new plasmid construction based on the inducible promoter of glucoamylase gene ( gla1 ) was used for the obtaining of new recombinant strains Penicillium verruculosum that secrete heterological xylanase E (XylE) P. canescens . New biocatalysts were developed which are XylEenriched cellulolytic enzyme preparations (EP). The content of XylE in recombinant EP varied within the optimal range from 11 to 24 % of the general protein pool at the preservation (in total) of the cellulase complex P. verruculosum . In comparison to EP prepared using the other expression systems and commercial preparations, the new EP demonstrated a higher hydrolytic activity with respect to polymers of plant substrates. For example, the efficiency of new EP glaX-17 to hydrolysis of aspenwood was higher by 13 % than that of the control EP agent and by 20 % than that of the commercial EP Accelerase Duet. In hydrolysis of wheat bran, glaX-17 was 35–43 % and 25 % more efficient than the control EP and the commercial EP Accelerase Duet, respectively. The efficiency of the new gla1 promoter for the production of EP (biocatalysts) at the preserved balanced cellulase complex of the strain and optimal yield of heterological XylE (that are necessary for complete hydrolysis of xylan-containing plant biomass) was demonstrated.
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