Abstract
A resonance light scattering technique to determine tinidazole in drugs was developed by tetraphenylboron sodium (TPB). Tinidazole was found to bind B(C6H5)−4 anion and transformed to tinidazole-TPB aggregate which displayed intense resonance scattering light. Effects of factors such as wavelength, acidity, stabilizers, and interferents on the RLS of tinidazole TPB were investigated in detail. The RLS intensity of the tinidazole-TPB suspension was obtained in sulfuric acid solution (pH = 1.44). The resonance scattering light intensity at the maximum RLS peak of 569.5 nm was linear to the concentration of tinidazole in the range of 10.0–30.0 μg mL−1 with a detection limit of 5.0 μg mL−1. Good results were also obtained with the recovery range of 95.13–106.76%. The method was applied to determine tinidazole in injections and tablets, showing high sensitivity and accuracy compared with the high performance liquid chromatography method (HPLC) according to Chinese Pharmacopoeia.
Highlights
Pasternack et al first established the Resonance light scattering (RLS) technique to study the biological macromolecules by means of an ordinary fluorescence spectrometer [3,4,5]
It can be seen that the RLS intensity of B(C6H5)4−Na is quite weak in the whole scanning wavelength region
Upon addition of trace amount of tinidazole to B(C6H5)4−Na solution, a remarkably enhanced RLS with a maximum peak at 569.5 nm was observed under the same conditions (Figure 2, c-g)
Summary
Pasternack et al first established the RLS technique to study the biological macromolecules by means of an ordinary fluorescence spectrometer [3,4,5]. Several analytical methods for tinidazole have been developed so far such as HPLC [19], LC-MS [20], capillary electrophoresis [21], spectrophotometry [22], voltammetry [23], and electrochemical methods [24, 25] Among these analytical methods, the voltammetry method according to Chinese Pharmacopoeia is popular and regarded relatively reliable for the determination of tinidazole.
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Journal of Automated Methods and Management in Chemistry
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
