Abstract

A molecular typing scheme developed for <i>P.pseudomallei</i> based on the patterns of restriction fragment length polymorphisms in rRNA genes, ribotype patterns, was evaluated in the study of strain relationships in clinical and epidemiological investigations. A series of human isolates from patients presenting with relapse of melioidosis and isolates from 4 outbreaks of animal melioidosis were ribotyped. Identical patterns among isolates from most of the relapse patients indicated the likely endogenous nature of these infections although re-infection from an exogenous source also occurs. Ribotyping identified the presence of a common strain among cases associated with the animal outbreaks and identified unrelated cases. Ribotyping is a useful epidemiological tool for melioidosis.

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