Abstract
This study aimed to investigate the antibacterial mechanism of glycinin basic peptide (GBP) against Staphylococcus aureus (S. aureus). Scanning electron microscope showed GBP treatment disrupted the integrity of S. aureus cells. And the leakages of Ca2+ and Mg2+ ions from bacterial cells further confirmed the membrane disruption by GBP. The electrophoresis of bacterial proteins and circular dichroism spectrum of bacterial DNA indicated GBP treatment caused the stress response of S. aureus and destroyed the bacterial DNA structure. Moreover, the results of DNA gel electrophoresis, reactive oxygen species (ROS) production and apoptosis analysis demonstrated the antibacterial mechanism of GBP was a result of increased intracellular ROS and blunted adaptive oxidative stress response in S. aureus. The excessive oxidative stress response ultimately led to the bacterial cell apoptosis. These suggested GBP mainly induced the cellular death of S. aureus by membrane disruption and ROS-mediated apoptosis pathway.
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