Abstract
Annona cherimola is an exotic fruit from the genus Annona, native to the Andean highlands in western South America. The cherimoya skin, flesh and juice were isolated and analyzed for antioxidant content using the oxygen radical absorbance capacity (ORAC) assay. The juice showed the highest antioxidant activity, while the flesh exhibited the lowest. The cherimoya skin, flesh and juice extracts were also incubated with Raji (Burkitt's Lymphoma) and HT-29 (colon cancer) cell lines, and the antioxidant uptake of cells was measured. Both cell lines, when subjected to cherimoya juice, showed the highest antioxidant uptake. The cells were then exposed to AAPH, a radical initiator, to simulate the conditions of cells under oxidative stress, and then subjected to cherimoya skin, flesh and juice extracts. Both cell lines absorbed more antioxidants after being pre-exposed to AAPH, indicating that cells under stress have the ability to import antioxidants.
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