Abstract
Mixtures of sugars and sugar alcohols, obtained by simultaneous isomerisation and hydrogenation glucose, have been analysed by ion-exchange chromatography. With a 25-cm column of Aminex A-27 and 0.11 M H 3BO 3/0.11 M Na 2B 4O 7 as eluant, a complete separation of glucose, fructose, glucitol, and mannitol is obtained in 50 min. The influence of various factors on the qualitative and quantitative results is given. For a boric acid eluant, the ratio Na 2B 4O 7/H 3BO 3 and the presence or acetic acid have an important influence on the elution order, and the presence of sodium chloride decreased the elution time.
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