Abstract
The AJ072 antibody against the human transferrin receptor labels the cell membrane of HeLa cells by surface immunofluorescence; AM236 does not.
Highlights
TfR (Transferrin receptor protein 1, CD71; Uniprot P02786) is a type II transmembrane glycoprotein that binds the iron-carrier glycoprotein transferrin (Tf)
After 3 washes (10 min) with PBS-BSA, cells were incubated during 5 min with DAPI (1:500, Molecular Probes D1306), washed twice with PBS-BSA and once with PBS, and mounted on slides (Menzel-Gläser, 76x26 mm) with Möwiol (Hoechst) + 2.5% (w/v) DABCO (Fluka 33480)
No staining was seen with AM236 antibody; this might be due to the fact that this antibody is poorly produced
Summary
TfR (Transferrin receptor protein 1, CD71; Uniprot P02786) is a type II transmembrane glycoprotein that binds the iron-carrier glycoprotein transferrin (Tf). After 3 washes (10 min) with PBS-BSA, cells were incubated during 5 min with DAPI (1:500, Molecular Probes D1306), washed twice with PBS-BSA and once with PBS, and mounted on slides (Menzel-Gläser, 76x26 mm) with Möwiol (Hoechst) + 2.5% (w/v) DABCO (Fluka 33480). Pictures were taken using a Zeiss LSM700 confocal microscope, with a 63x Neofluar oil immersion objective.
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