Abstract
The double-stranded RNA genome of cytoplasmic polyhedrosis virus contains ten kinds of segments, namely twenty RNA terminals. The 3′-termini of these RNA segments were studied by 3H-labeling the terminal ribose moiety, with periodate oxidation and successive reduction using tritiated borohydride. None of the 3′-termini of ten RNA segments were phosphorylated as all were labeled to the same extent. The 3′-terminal nucleosides were determined as 50% cytidine (ten termini) and 50% uridine (ten termini) by analysis of nucleoside tri-alcohols obtained from the ribonuclease T 2 (or alkali) digest of the 3H-labeled RNA. In a 3H-labeled RNA preparation, radioactive material other than RNA was found. The data suggest that this material may be a complex consisting of polysaccharide and protein.
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