Abstract
The human β-actin promoter has been widely used to drive expression of genes of interest in mammalian cell lines and transgenic mice. The original form of the human β-actin expression vector contains upstream sequences, 5′UTR (untranslated region) and intron 1 from the β-actin gene linked to a three restriction site polylinker and SV40 (Simian Virus 40) 3′UTR. We have modified this vector now to contain the highly conserved β-actin 3′UTR plus flanking region which replaces the SV40 sequences. An additional modification has removed the mRNA peripheral localization sequences present in the β-actin 3′UTR. The new vectors also contain an improved polylinker. The activity of these two new vectors has been compared with that of the original vector and that of a vector using the popular cytomegalovirus (CMV) promoter. Mouse C2 myoblasts were transfected with each vector driving expression of enhanced green fluorescent protein (EGFP) and analyzed for EGFP mRNA levels. We find that both new vectors drive twice the level of mRNA accumulation of the original vector and over 30-times that of the CMV promoter. This suggests that these new vectors will provide a substantial elevation in levels of expression by virtue of inclusion of the β-actin 3′UTR plus flanking region.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
