Abstract
BackgroundColonization of Trichoderma spp. is essential for exerting their beneficial functions on the plant. However, the interactions between Trichoderma spp. and plant roots are still not completely understood. The aim of this study was to investigate how TgSWO affect Trichoderma guizhouense to establish themselves in the plant rhizosphere and promote plant growth. In this study, we deeply analyzed the molecular mechanism by which the functional characterization of the TgSWO by expressing different functional region deletion proteins (FRDP) of TgSWO.ResultsRoot scanning analysis results showed that TgSWO could dramatically increase root density and promote growth. In addition, we also found that TgSWO could expand root cell walls, subsequently increase root colonization. Moreover, knockout of TgSWO mutants (KO) or overexpression of TgSWO mutants (OE) produced greatly reduced or increased the number of cucumber root, respectively. To clarify the molecular mechanism of TgSWO in plant-growth-promotion, we analyzed the ability of different FRDP to expand the root cell wall. The root cell wall architecture were considerably altered when treated by ΔCBD protein (the TgSWO gene of lacking in the CBD domain was cloned and heterologously expressed), in correlation with the present YoaJ domain of TgSWO. In contrast, neither the expansion of cell walls nor the increase of roots was detectable in ΔYoaJ protein.ConclusionsOur results emphasize the YoaJ domain is the most critical functional area of TgSWO during the alteration of cell wall architecture. Simultaneously, the results obtained in this study also indicate that TgSWO might play a plant-growth-promotion role in the Trichoderma-plant interactions by targeting the root cell wall.
Highlights
Colonization of Trichoderma spp. is essential for exerting their beneficial functions on the plant
TgSWO was induced by the cellulose component of the root To study whether TgSWO is involved in the interaction between Trichoderma and cucumber, NJAU4742 strain was inoculated into different treatments and grew for 5 days
The results obtained here suggest that the TgSWO gene could be induced by the cellulose component of the root and that it might participate in the promotion of growth in cucumber
Summary
Colonization of Trichoderma spp. is essential for exerting their beneficial functions on the plant. The aim of this study was to investigate how TgSWO affect Trichoderma guizhouense to establish themselves in the plant rhizosphere and promote plant growth. Trichoderma spp. are some of the most widely distributed plant growth-promoting microbes (PGPM) in agroecosystems worldwide [1, 2], and they exhibit extremely high levels of ecological adaptability through their symbiotic. Inoculation of Trichoderma spp. have been reported to affect the maize root system architecture, enhancing root biomass production and increasing root hair development, which may be Trichoderma spp. colonize the entire root system and persist for the whole lifespan of this crop. Extensive studies about the role of Trichoderma spp. in promoting plant growth have been conducted [14], the exact molecular mechanisms that govern the recognition and association between Trichoderma and plant roots are still far from conclusive
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