Abstract
Clematis heracleifolia is a beautiful garden plant, and both the whole plant and its root are important in traditional Chinese medicine. Polyploids are usually more valuable because they exhibit increased biomass and content of effective compounds for medicinal plants. This research aimed to induce tetraploidy in C. heracleifolia by the apical meristem method using 0.2% colchicine. Apical buds were induced under three treatments (24, 48 and 72 h) to explore the best treatment time. Cytology identification was accomplished for the obtained mutants and diploid controls. It showed that the highest mutation rate of up to 80% was obtained with the 48 h treatment. The leaves of mutants were much broader, thicker, and showed lower leaf index compared with controls by morphology examination. There were significant differences between tetraploids and controls for stoma size and stoma frequency at the 0.01 or 0.05 levels. The chromosome number of most mutants was 32 (2n = 4x), while the chromosome number of diploid...
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
